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Matt Brunke, DVM, CCRP, CVPP, CVA Diplomate, American College of Veterinary Sports Medicine and Rehabilitation

NUTRITIONAL SUPPLEMENTS FOR ANIMAL ATHLETES, WHAT KEEPS JOINT HAPPY?:
PART FOUR

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Cannabis vs. Hemp
- Cannabis Sativa
- Recreational andmedical drug
- Restricted substance
- Leaves and flowers
- THC >0.3%
- Hemp - Cannabis
- Industrial
- Tall, sturdy stalks
- Paper, textiles, health food, fuel, biodegradable plastics
- THC<0.3%

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CBDs - Cannabidioils
- December 2018 - Hemp Farming Act
- Hemp legal - as “agricultural commodity”
- Removed from Schedule 1 status
- Under FDA jurisdiction
- Currently DSHEA - supplement act for humans; NOT animals
- Can’t discuss specific chemicals or have drug claims on label
- State laws for vet med - will vary

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CBD - Cannnabidiol

Single-Dose Pharamacokinetics and Preliminary Safety Assessment with Use of CBD-Rich Hemp Nutraceutical in Healthy Dogs and Cats.

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CBD - But how safe is it?

- Dogs given 25mg/kg CBD - no issues
- In a long term study (56 weeks)

Species- specific Susceptibility to Cannabis-Induced Convulsions

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Are they all the same?

- http://www.cannabissciencetech.com/article/analysis-veterinary-hemp-based-oils-
product-integrity-lcms/page/0/3

Analysis of Veterinary Hemp-Based Oils for Product Integrity by LC/MS

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Abstract/Synopsis

Pet owners continue to seek alternative therapies for pain relief in their favorite companion animals. Instead of routine nonsteroidal anti-inflammatory drug (NSAID) treatment, there is a trend toward trying hemp-based products rich in cannabinoids. There are increasing number of companies producing hemp-based oils enriched with various mixtures of cannabinoidsfor pain and other maladies inducing seizures, cancer and anxiety. These products post labels attesting to the composition in the products, usually labelling for the cannabidiol (CBD) concentration in the products. But in the absence of regulatory control we questioned how accurate the information is on the label. The chemical composition of 13 commercially available oils intended for veterinary or a crossover of human and veterinary use was determined by selected ion monitoring liquid chromatography/mass spectrometry (SIM LC/MS). It was found that many of the labels were inaccurate regarding the cannabidiol concentration and/or the presence of other cannabinoids. In general, the label o most of the medicinal veterinary samples indicated higher levels of CBD than found in these studies by 20% or more. The precision and accuracy of SIM LC/MS analysis of the samples fell within the acceptable limits of regulated bioanalysis guidelines of +/- 15%.

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Table II. Summary of veterinary CBD oil concentration for the six cannabinoids measured in samples 1-13. Concentration are mg/mL of indicated cannabinoids. It should be noted that sample 6 was crude hemp leaf extract without subsequent sample cleanup or concentration

Vet CBD Oil CBDA CBG CBD CBN THC THCA
1 0.005 0.070 13.4 0.078 0.347 0.107
2 0.007 0.051 10.6 0.005 0.194 0.026
3 0.001 N/D 1.65 0.030 0.023 0.075
4 N/D N/D 6.51 0.003 0.001 0.088
5 0.042 0.026 4.34 0.018 0.103 0.076
6 0.153 N/D 0.88 0.002 0.014 0.244
7 1.42 0.061 5.76 N/D 0.205 0.049
8 0.291 0.274 15.5 0.062 0.348 0.180
9 0.005 0.147 8.82 0.009 0.278 N/D
10 N/D 0.102 11.6 0.017 0.352 N/D
11 N/D 0.018 7.86 N/D 0.049 0.079
12 11.8 0.372 27.5 0.005 1.29 0.693
13 0.123 0.116 13.9 N/D 0.374 0.032

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Table III. A listing of the indicated concentrations on the product labels of the 13 veterinary medicinal oils studied. The lack of uniform guidelines or practices for labelling medicinal CBD oils leads to considerable confusion for customers.
Sample Numbers CBD label
1 25 mg hemp extract/ml
2 N/A
3 200 mg/oz
4 10 mg/mL
5 4.2 mg/mL
6 56 mg hemp leaf extract/ml
7 250 mg cannabinoids
8 5 mg hemp oil/0.5 ml
9 350 mg/30 ml
10 225 mg total in 15 ml
11 150 mg/18 ml
12 website 3000 mg/60 ml
13 8 mg/mL

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CBD content

CBD (mg/mL)
Vet CBD Oil

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THC Content

THC (ppm)
Vet CBD Oil

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Conclusions
- How accurate is the label?
- Most were not
- Varied in CBD by 20% or more
- Ask for their analytical composition data

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Conclusions
- Feeding enhances absorption
- 2mg/kg BID is starting point and safe

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CBD
- Cannabinoid compound in hemp
- Cannabinoid receptors in CNS and PNS
- Decreases pain
- Inhibits spinal nociceptive processing?
- Decreases inflammation
- Decreases IL-1 and TNF-alpha
- Increases Il-10 (anti-inflam)